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Part I covers all the important aspects of plant tissue culture, viz. nutrition media, micropropagation, organ culture, cell suspension culture, haploid culture, protoplast isolation and fusion, secondary metabolite production, somaclonal variation and cryopreservation.
Part II covers chapters on genetic material, organization of DNA in the genome and basic techniques involved for understanding of recombinant DNA technology.
Part III deals with different aspects on rDNA technology which covers gene cloning, isolation of plant genes, transposons and gene tagging, in vitro mutagenesis, PCR, molecular markers and marker assisted selection, gene transfer methods, chloroplast and mitochondrion DNA transformation, genomics and bioinformatics. Genomics explores functional and structural genomics, proteomics, metabolomics, sequencing status of different organisms and DNA chip technology. Application of biotechnology has been discussed as transgenics in crop improvement and impact of recombinant DNA technology mainly in relation to biotech crops. A chapter on biosafety concerns, regulatory framework and issues and controversies arisen at different times on biosafety have been given. Various forms of intellectual properties, plant breeders rights, biodiversity and some examples of patents, which generated a lot of debate have also been discussed in a chapter on intellectual property rights.
Part IV, introduced new in this edition, deals with genome editing aspects. It covers chapters on genome editing tools, applications of this approach in crop improvement and genome editing regulatory policy in different countries. Protocols are discussed for conducting laboratory exercises that are essential at the graduate level. Application of techniques and their impact on crop biotechnology have been emphasized in the book.
Additional Information
| Publisher | CBS Publishers & Distributors Pvt. Ltd |
|---|---|
| Binding | Paperback |
| Size | 18.5cm×24cm×3cm |
| Year | 2024 |
| Edition | 4th Edition |
| Pages | 800 |
| Author | HS Chawla |
| Subject | Life Sciences, botany, biotechnology, microbiology |
| About the Author | HS Chawla PhD has more than 40 years of diversified and rich experience in teaching and research on biotechnology, genetics and intellectual property rights. He has done extensive work on in vitro culture, genetic transformation of plants, molecular markers and supervised the research of numerous MSc students and PhD. He has published nine books and manuals on biotechnology and genetics aspects, one book on intellectual property rights, three booklets on intellectual property rights,and has to his credit more than 100 research papers published in journals of repute. Dr Chawla has availed fellowships offered by various governments: Germany (1985-87; 1997) and Canada (1993- 1995) and visited many countries.He was the Nodal officer of Patent Information Centre for managing the IPR affairs of the Uttarakhand State and the CEO of Intellectual Property Management Centre of the GB Pant University. He is an active resource person as well as an advisor for multiple IPR organizations in agriculture and biotechnology aspects. He has been a member of the executive committee and fellow of Indian Society of Genetics, member of Plant Tissue Culture Association (India), is a member|fellow|editorial board member of several professional societies, and member of various government bodies. |